Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method us...
Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites
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Publisher
England: BioMed Central Ltd
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English
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England: BioMed Central Ltd
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16S rDNA-PCR for the identification of a bacterial species is an established method. However, the DNA extraction reagents as well as the PCR reagents may contain residual bacterial DNA, which consequently generates false-positive PCR results. Additionally, previously used methods are frequently time-consuming. Here, we describe the results obtained...
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Full title
Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites
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TN_cdi_doaj_primary_oai_doaj_org_article_cf27f0e5da274600ac817ae178d9fbaa
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_cf27f0e5da274600ac817ae178d9fbaa
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ISSN
1471-2180
E-ISSN
1471-2180
DOI
10.1186/s12866-022-02542-w
