Pooled extracellular receptor-ligand interaction screening using CRISPR activation
Pooled extracellular receptor-ligand interaction screening using CRISPR activation
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Full title
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Publisher
England: BioMed Central Ltd
Journal title
Language
English
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Publisher
England: BioMed Central Ltd
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Scope and Contents
Contents
Extracellular interactions between cell surface receptors are necessary for signaling and adhesion but identifying them remains technically challenging. We describe a cell-based genome-wide approach employing CRISPR activation to identify receptors for a defined ligand. We show receptors for high-affinity antibodies and low-affinity ligands can be...
Alternative Titles
Full title
Pooled extracellular receptor-ligand interaction screening using CRISPR activation
Authors, Artists and Contributors
Author / Creator
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Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_d3fc6ae9c91a4286ae9804008897fd13
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_d3fc6ae9c91a4286ae9804008897fd13
Other Identifiers
ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-018-1581-3
