An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure
An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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The mechanism of phagophore closure remains unclear due to technical limitations in distinguishing unclosed and closed autophagosomal membranes. Here, we report the HaloTag-LC3 autophagosome completion assay that specifically detects phagophores, nascent autophagosomes, and mature autophagic structures. Using this assay, we identify the endosomal s...
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An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure
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TN_cdi_doaj_primary_oai_doaj_org_article_dc0bd3a5e9b64caca02caa285ccc24af
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_dc0bd3a5e9b64caca02caa285ccc24af
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-018-05254-w
