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Efficient isolation of protoplasts from rice calli with pause points and its application in transien...

Efficient isolation of protoplasts from rice calli with pause points and its application in transien...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_dff7cd4baaee4dea962be33ec0a97159

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

About this item

Full title

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Publisher

London: BioMed Central Ltd

Journal title

Plant methods, 2020-11, Vol.16 (1), p.1-151, Article 151

Language

English

Formats

Publication information

Publisher

London: BioMed Central Ltd

More information

Scope and Contents

Contents

An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings or cell suspension culture, preparation of those donor tissues can be inefficient, time-consuming,...

Alternative Titles

Full title

Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_dff7cd4baaee4dea962be33ec0a97159

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_dff7cd4baaee4dea962be33ec0a97159

Other Identifiers

ISSN

1746-4811

E-ISSN

1746-4811

DOI

10.1186/s13007-020-00692-4

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