Efficient isolation of protoplasts from rice calli with pause points and its application in transien...
Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays
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London: BioMed Central Ltd
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English
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London: BioMed Central Ltd
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An efficient in vivo transient transfection system using protoplasts is an important tool to study gene expression, metabolic pathways, and multiple mutagenesis parameters in plants. Although rice protoplasts can be isolated from germinated seedlings or cell suspension culture, preparation of those donor tissues can be inefficient, time-consuming,...
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Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays
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TN_cdi_doaj_primary_oai_doaj_org_article_dff7cd4baaee4dea962be33ec0a97159
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_dff7cd4baaee4dea962be33ec0a97159
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ISSN
1746-4811
E-ISSN
1746-4811
DOI
10.1186/s13007-020-00692-4