Long-read sequencing and genome assembly of natural history collection samples and challenging speci...
Long-read sequencing and genome assembly of natural history collection samples and challenging specimens
About this item
Full title
Author / Creator
Bein, Bernhard , Chrysostomakis, Ioannis , Arantes, Larissa S , Brown, Tom , Gerheim, Charlotte , Schell, Tilman , Schneider, Clément , Leushkin, Evgeny , Chen, Zeyuan , Sigwart, Julia , Gonzalez, Vanessa , Wong, Nur Leena W S , Santos, Fabricio R , Blom, Mozes P K , Mayer, Frieder , Mazzoni, Camila J , Böhne, Astrid , Winkler, Sylke , Greve, Carola and Hiller, Michael
Publisher
England: BioMed Central Ltd
Journal title
Language
English
Formats
Publication information
Publisher
England: BioMed Central Ltd
Subjects
More information
Scope and Contents
Contents
Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase to overcom...
Alternative Titles
Full title
Long-read sequencing and genome assembly of natural history collection samples and challenging specimens
Authors, Artists and Contributors
Author / Creator
Chrysostomakis, Ioannis
Arantes, Larissa S
Brown, Tom
Gerheim, Charlotte
Schell, Tilman
Schneider, Clément
Leushkin, Evgeny
Chen, Zeyuan
Sigwart, Julia
Gonzalez, Vanessa
Wong, Nur Leena W S
Santos, Fabricio R
Blom, Mozes P K
Mayer, Frieder
Mazzoni, Camila J
Böhne, Astrid
Winkler, Sylke
Greve, Carola
Hiller, Michael
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_e879aceac2c848f0bab2f68949557e8e
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_e879aceac2c848f0bab2f68949557e8e
Other Identifiers
ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-025-03487-9
