Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based bios...
Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Near-infrared (NIR) probes are highly sought after as fluorescent tags for multicolor cellular and in vivo imaging. Here we develop small NIR fluorescent nanobodies, termed NIR-Fb
LAG16
and NIR-Fb
LAG30
, enabling background-free visualization of various GFP-derived probes and biosensors. We also design a red-shifted variant, NIR-Fb
...
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Full title
Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation
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TN_cdi_doaj_primary_oai_doaj_org_article_e98155c0e6a34182a01932e1e57b2510
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_e98155c0e6a34182a01932e1e57b2510
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-024-51857-x
