An optimized CRISPR/Cas9 approach for precise genome editing in neurons
An optimized CRISPR/Cas9 approach for precise genome editing in neurons
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Author / Creator
Publisher
England: eLife Science Publications, Ltd
Journal title
Language
English
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Publisher
England: eLife Science Publications, Ltd
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Contents
The efficient knock-in of large DNA fragments to label endogenous proteins remains especially challenging in non-dividing cells such as neurons. We developed
T
argeted
K
nock-
I
n with
T
wo (TKIT) guides as a novel CRISPR/Cas9 based approach for efficient, and precise, genomic knock-in. Through targeting non-coding regions T...
Alternative Titles
Full title
An optimized CRISPR/Cas9 approach for precise genome editing in neurons
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TN_cdi_doaj_primary_oai_doaj_org_article_f28c7ab31bc7495cb8cd289fa0be3e67
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_f28c7ab31bc7495cb8cd289fa0be3e67
Other Identifiers
ISSN
2050-084X
E-ISSN
2050-084X
DOI
10.7554/eLife.65202
