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Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the...

Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_f2c43d218656488b8e89f1ef1d575233

Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

About this item

Full title

Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

Publisher

England: BioMed Central Ltd

Journal title

BMC research notes, 2011-05, Vol.4 (1), p.162-162, Article 162

Language

English

Formats

Publication information

Publisher

England: BioMed Central Ltd

More information

Scope and Contents

Contents

The accuracy of quantitative real-time RT-PCR (qRT-PCR) is often influenced by experimental artifacts, resulting in erroneous expression profiles of target genes. The practice of employing normalization using a reference gene significantly improves reliability and its applicability to molecular biology. However, selection of an ideal reference gene...

Alternative Titles

Full title

Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_f2c43d218656488b8e89f1ef1d575233

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_f2c43d218656488b8e89f1ef1d575233

Other Identifiers

ISSN

1756-0500

E-ISSN

1756-0500

DOI

10.1186/1756-0500-4-162

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