Streamlined protein expression and purification using cleavable self-aggregating tags
Streamlined protein expression and purification using cleavable self-aggregating tags
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Author / Creator
Xing, Lei , Wu, Wei , Zhou, Bihong and Lin, Zhanglin
Publisher
England: BioMed Central Ltd
Journal title
Language
English
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Publication information
Publisher
England: BioMed Central Ltd
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Scope and Contents
Contents
Recombinant protein expression and purification remains a fundamental issue for biotechnology. Recently we found that two short self-assembling amphipathic peptides 18A (EWLKAFYEKVLEKLKELF) and ELK16 (LELELKLKLELELKLK) can induce the formation of active protein aggregates in Escherichia coli (E. coli), in which the target proteins retain high enzym...
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Full title
Streamlined protein expression and purification using cleavable self-aggregating tags
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TN_cdi_doaj_primary_oai_doaj_org_article_f6612af6166648a4937a35bfd839f2a5
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_f6612af6166648a4937a35bfd839f2a5
Other Identifiers
ISSN
1475-2859
E-ISSN
1475-2859
DOI
10.1186/1475-2859-10-42