Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex
Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex
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Publisher
England: BioMed Central Ltd
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Language
English
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Publisher
England: BioMed Central Ltd
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CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome.
We tested two gene disruption methods in T. reesei using CRISPR/C...
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Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex
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TN_cdi_doaj_primary_oai_doaj_org_article_f7d87169d3304977938f6fe56c5a87e5
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_f7d87169d3304977938f6fe56c5a87e5
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ISSN
1472-6750
E-ISSN
1472-6750
DOI
10.1186/s12896-018-0498-y
