Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA b...
Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA binding to avidin and streptavidin
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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The high affinity (KD ~ 10-15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume that the association rate constant (kon) is approximately diffusion limited (109 M-1s-1) but recent s...
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Full title
Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA binding to avidin and streptavidin
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TN_cdi_doaj_primary_oai_doaj_org_article_fb077640e6a6488c9760c8c1768cf7ce
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_fb077640e6a6488c9760c8c1768cf7ce
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0204194
