One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free...
One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Recently developed transgenic techniques to explore and exploit the metabolic potential of microalgae present several drawbacks associated with the delivery of exogenous DNA into the cells and its subsequent integration at random sites within the genome. Here, we report a highly efficient multiplex genome-editing method in the diatom
Phaeodactyl...
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Full title
One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
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TN_cdi_doaj_primary_oai_doaj_org_article_fc8706bd038248e3be9ac9620f125fd2
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_fc8706bd038248e3be9ac9620f125fd2
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-018-06378-9