The effects of singlet oxygen (
1
O
2
) transfer to bacteria attached on phytodetritus were investigated under laboratory-controlled conditions. For this purpose, a nonaxenic culture of
Emiliania huxleyi
in late stationary phase was studied for bacterial viability. Our results indicated that only 9 ± 3 % of attached bacteria were alive compared to 46 ± 23 % for free bacteria in the
E. huxleyi
culture. Apparently, under conditions of low irradiance (36 W m
−2
), during the culture, the cumulative dose received (22,000 kJ m
−2
) was sufficiently important to induce an efficient
1
O
2
transfer to attached bacteria during the senescence of
E. huxleyi
cells. At this stage, attached bacteria appeared to be dominated by pigmented bacteria (
Maribacter
,
Roseobacter
,
Roseovarius
), which should resist to
1
O
2
stress probably due to their high contents of carotenoids. After subsequent irradiation of the culture until fully photodegradation of chlorophyll, DGGE analyses showed that the diversity of bacteria attached to
E. huxleyi
cells is modified by light. Photooxidative alterations of bacteria were confirmed by the increasing amounts of
cis
-vaccenic photoproducts (bacterial marker) per bacteria observed during irradiation time. Interestingly, preliminary chemotaxis experiments showed that
Shewanella oneidensis
considered here as a model of motile bacteria was attracted by phytodetritus producing or not
1
O
2
. This lack of repulsive effects could explain the high mortality rate of bacteria measured on
E. huxleyi
cells.