Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM
Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light...
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Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM
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TN_cdi_hal_primary_oai_HAL_hal_02270788v1
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_hal_primary_oai_HAL_hal_02270788v1
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/s41592-019-0462-3
