Gibson assembly : an easy way to clone potyviral full-length infectious cDNA clones ex pressing an e...
Gibson assembly : an easy way to clone potyviral full-length infectious cDNA clones ex pressing an ectopic VPg
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Publisher
BioMed Central
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Language
English
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BioMed Central
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Abstract Background Approaches to simplify and accelerate the construction of full-length infectious cDNA clones for plant potyviruses have been described, based on cloning strategies involving in vitro ligation or homologous recombination in yeast. In the present study, we developed a faster and more efficient in vitro recombination system using G...
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Full title
Gibson assembly : an easy way to clone potyviral full-length infectious cDNA clones ex pressing an ectopic VPg
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TN_cdi_hal_primary_oai_HAL_hal_02636073v1
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_hal_primary_oai_HAL_hal_02636073v1
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ISSN
1743-422X
E-ISSN
1743-422X
DOI
10.1186/s12985-015-0315-3
