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Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach

Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_hal_primary_oai_HAL_hal_03032928v1

Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach

About this item

Full title

Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach

Publisher

Nature Publishing Group

Journal title

Scientific reports, 2019-12, Vol.9 (1)

Language

English

Formats

Publication information

Publisher

Nature Publishing Group

More information

Scope and Contents

Contents

Because quantitative reverse transcription PCR (RT-qPCR) gene expression data are compositional, amounts of quantified RNAs must be normalized using reference genes. However, the two most used methods to select reference genes (NormFinder and geNorm) ignore the compositional nature of RT-qPCR data, and often lead to different results making reliabl...

Alternative Titles

Full title

Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_hal_primary_oai_HAL_hal_03032928v1

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_hal_primary_oai_HAL_hal_03032928v1

Other Identifiers

ISSN

2045-2322

E-ISSN

2045-2322

DOI

10.1038/s41598-019-52217-2

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