Efficient Construction of a Large Nonimmune Phage Antibody Library: The Production of High-Affinity...
Efficient Construction of a Large Nonimmune Phage Antibody Library: The Production of High-Affinity Human Single-Chain Antibodies to Protein Antigens
About this item
Full title
Author / Creator
Publisher
United States: National Academy of Sciences of the United States of America
Journal title
Language
English
Formats
Publication information
Publisher
United States: National Academy of Sciences of the United States of America
Subjects
More information
Scope and Contents
Contents
A large library of phage-displayed human single-chain Fv antibodies (scFv), containing 6.7 × 109members, was generated by improving the steps of library construction. Fourteen different protein antigens were used to affinity select antibodies from this library. A panel of specific antibodies was isolated with each antigen, and each panel contained...
Alternative Titles
Full title
Efficient Construction of a Large Nonimmune Phage Antibody Library: The Production of High-Affinity Human Single-Chain Antibodies to Protein Antigens
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_jstor_primary_45348
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_jstor_primary_45348
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.95.11.6157
