Improved bacterial recombineering by parallelized protein discovery

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_osti_scitechconnect_1631183

Improved bacterial recombineering by parallelized protein discovery

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Author / Creator

Wannier, Timothy M. , Nyerges, Akos , Kuchwara, Helene M. , Czikkely, Márton , Balogh, Dávid , Filsinger, Gabriel T. , Borders, Nathaniel C. , Gregg, Christopher J. , Lajoie, Marc J. , Rios, Xavier , Pál, Csaba , Church, George M. and Harvard Medical School, Boston, MA (United States)

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United States: National Academy of Sciences

Journal title

Proceedings of the National Academy of Sciences - PNAS, 2020-05

Language

English

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United States: National Academy of Sciences

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Contents

Exploiting bacteriophage-derived homologous recombination processes has offered precise, multiplex editing of microbial genomes and the construction of billions of customized genetic variants in a single day. The techniques that enable this, multiplex automated genome engineering (MAGE) and directed evolution with random genomic mutations (DIvERGE)...