In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
About this item
Full title
Author / Creator
Karim, Ashty S. , Dudley, Quentin M. , Juminaga, Alex , Yuan, Yongbo , Crowe, Samantha A. , Heggestad, Jacob T. , Garg, Shivani , Abdalla, Tanus , Grubbe, William S. , Rasor, Blake J. , Coar, David N. , Torculas, Maria , Krein, Michael , Liew, FungMin (Eric) , Quattlebaum, Amy , Jensen, Rasmus O. , Stuart, Jeffrey A. , Simpson, Sean D. , Köpke, Michael , Jewett, Michael C. and Northwestern Univ., Evanston, IL (United States)
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
Formats
Publication information
Publisher
New York: Nature Publishing Group US
Subjects
More information
Scope and Contents
Contents
The design and optimization of biosynthetic pathways for industrially relevant, non-model organisms is challenging due to transformation idiosyncrasies, reduced numbers of validated genetic parts and a lack of high-throughput workflows. Here we describe a platform for in vitro prototyping and rapid optimization of biosynthetic enzymes (iPROBE) to a...
Alternative Titles
Full title
In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design
Authors, Artists and Contributors
Author / Creator
Dudley, Quentin M.
Juminaga, Alex
Yuan, Yongbo
Crowe, Samantha A.
Heggestad, Jacob T.
Garg, Shivani
Abdalla, Tanus
Grubbe, William S.
Rasor, Blake J.
Coar, David N.
Torculas, Maria
Krein, Michael
Liew, FungMin (Eric)
Quattlebaum, Amy
Jensen, Rasmus O.
Stuart, Jeffrey A.
Simpson, Sean D.
Köpke, Michael
Jewett, Michael C.
Northwestern Univ., Evanston, IL (United States)
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_osti_scitechconnect_1659129
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_osti_scitechconnect_1659129
Other Identifiers
ISSN
1552-4450
E-ISSN
1552-4469
DOI
10.1038/s41589-020-0559-0
