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High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis Reveals a Glucose-Response Protei...

High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis Reveals a Glucose-Response Protei...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_osti_scitechconnect_6875096

High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis Reveals a Glucose-Response Protein of 65 kDa in Pancreatic Islet Cells

About this item

Full title

High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis Reveals a Glucose-Response Protein of 65 kDa in Pancreatic Islet Cells

Publisher

Washington, DC: National Academy of Sciences of the United States of America

Journal title

Proceedings of the National Academy of Sciences - PNAS, 1990-07, Vol.87 (14), p.5494-5498

Language

English

Formats

Publication information

Publisher

Washington, DC: National Academy of Sciences of the United States of America

More information

Scope and Contents

Contents

High-resolution two-dimensional PAGE was used to search for glucose-response proteins in isolated pancreatic islets that were labeled with [35S]methionine at ambient glucose concentrations of 0-18 mM. A 65-kDa protein, isoelectric focusing point of approximately 6.6-7.0, was discovered that showed at least a 20-fold stimulation of radiolabeling whe...

Alternative Titles

Full title

High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis Reveals a Glucose-Response Protein of 65 kDa in Pancreatic Islet Cells

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_osti_scitechconnect_6875096

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_osti_scitechconnect_6875096

Other Identifiers

ISSN

0027-8424

E-ISSN

1091-6490

DOI

10.1073/pnas.87.14.5494