Condensation of an additive-free cell extract to mimic the conditions of live cells
Condensation of an additive-free cell extract to mimic the conditions of live cells
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Publisher
United States: Public Library of Science
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Language
English
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Publisher
United States: Public Library of Science
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Scope and Contents
Contents
The cellular environment differs from that of reconstituted materials mainly because of the presence of highly condensed biomacromolecules. To mimic the environment and conditions in living cells, we developed a method to prepare additive-free, highly concentrated cell extracts. First, we verified the requirement for specific salts and buffers for...
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Full title
Condensation of an additive-free cell extract to mimic the conditions of live cells
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TN_cdi_plos_journals_1289067309
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1289067309
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0054155