Quick and Clean Cloning: A Ligation-Independent Cloning Strategy for Selective Cloning of Specific P...
Quick and Clean Cloning: A Ligation-Independent Cloning Strategy for Selective Cloning of Specific PCR Products from Non-Specific Mixes
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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Scope and Contents
Contents
We have developed an efficient strategy for cloning of PCR products that contain an unknown region flanked by a known sequence. As with ligation-independent cloning, the strategy is based on homology between sequences present in both the vector and the insert. However, in contrast to ligation-independent cloning, the cloning vector has homology wit...
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Full title
Quick and Clean Cloning: A Ligation-Independent Cloning Strategy for Selective Cloning of Specific PCR Products from Non-Specific Mixes
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TN_cdi_plos_journals_1304981695
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1304981695
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0020556
