Practical Tools to Implement Massive Parallel Pyrosequencing of PCR Products in Next Generation Mole...
Practical Tools to Implement Massive Parallel Pyrosequencing of PCR Products in Next Generation Molecular Diagnostics
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Author / Creator
De Leeneer, Kim , De Schrijver, Joachim , Clement, Lieven , Baetens, Machteld , Lefever, Steve , De Keulenaer, Sarah , Van Criekinge, Wim , Deforce, Dieter , Van Nieuwerburgh, Filip , Bekaert, Sofie , Pattyn, Filip , De Wilde, Bram , Coucke, Paul , Vandesompele, Jo , Claes, Kathleen and Hellemans, Jan
Publisher
United States: Public Library of Science
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Language
English
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Publisher
United States: Public Library of Science
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Scope and Contents
Contents
Despite improvements in terms of sequence quality and price per basepair, Sanger sequencing remains restricted to screening of individual disease genes. The development of massively parallel sequencing (MPS) technologies heralded an era in which molecular diagnostics for multigenic disorders becomes reality. Here, we outline different PCR amplifica...
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Full title
Practical Tools to Implement Massive Parallel Pyrosequencing of PCR Products in Next Generation Molecular Diagnostics
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TN_cdi_plos_journals_1308858148
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1308858148
Other Identifiers
ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0025531
