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Advantages and limitations of different p62-based assays for estimating autophagic activity in Droso...

Advantages and limitations of different p62-based assays for estimating autophagic activity in Droso...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1326547261

Advantages and limitations of different p62-based assays for estimating autophagic activity in Drosophila

About this item

Full title

Advantages and limitations of different p62-based assays for estimating autophagic activity in Drosophila

Publisher

United States: Public Library of Science

Journal title

PloS one, 2012-08, Vol.7 (8), p.e44214-e44214

Language

English

Formats

Publication information

Publisher

United States: Public Library of Science

More information

Scope and Contents

Contents

Levels of the selective autophagy substrate p62 have been established in recent years as a specific readout for basal autophagic activity. Here we compared different experimental approaches for using this assay in Drosophila larvae. Similar to the more commonly used western blots, quantifying p62 dots in immunostained fat body cells of L3 stage lar...

Alternative Titles

Full title

Advantages and limitations of different p62-based assays for estimating autophagic activity in Drosophila

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_plos_journals_1326547261

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1326547261

Other Identifiers

ISSN

1932-6203

E-ISSN

1932-6203

DOI

10.1371/journal.pone.0044214

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