PFunkel: Efficient, Expansive, User-Defined Mutagenesis
PFunkel: Efficient, Expansive, User-Defined Mutagenesis
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United States: Public Library of Science
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Language
English
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Publisher
United States: Public Library of Science
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Scope and Contents
Contents
We introduce PFunkel, a versatile method for extensive, researcher-defined DNA mutagenesis using a ssDNA or dsDNA template. Once the template DNA is prepared, the method can be completed in a single day in a single tube, and requires no intermediate DNA purification or sub-cloning. PFunkel can be used for site-directed mutagenesis at an efficiency...
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Full title
PFunkel: Efficient, Expansive, User-Defined Mutagenesis
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Author / Creator
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TN_cdi_plos_journals_1327174450
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1327174450
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0052031
