Express Your LOV: An Engineered Flavoprotein as a Reporter for Protein Expression and Purification
Express Your LOV: An Engineered Flavoprotein as a Reporter for Protein Expression and Purification
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function. Specifically, we used iLOV, an enhanced and more photostable variant of LOV. A pET-based plasmid for protein expression was constructed, encoding a C terminal iLOV-octahisti...
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Full title
Express Your LOV: An Engineered Flavoprotein as a Reporter for Protein Expression and Purification
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TN_cdi_plos_journals_1327231086
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1327231086
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0052962
