Expansion of IgG+ B-Cells during Mitogen Stimulation for Memory B-Cell ELISpot Analysis Is Influence...
Expansion of IgG+ B-Cells during Mitogen Stimulation for Memory B-Cell ELISpot Analysis Is Influenced by Size and Composition of the B-Cell Pool
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United States: Public Library of Science
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English
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United States: Public Library of Science
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The memory B-cell (MBC) ELISpot assay is the main technique used to measure antigen-specific MBCs as a readout of humoral immune memory. This assay relies on the ability of MBCs to differentiate into antibody-secreting cells (ASC) upon polyclonal stimulation. The total number of IgG+ ASCs generated by mitogen-stimulation is often used as a referenc...
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Expansion of IgG+ B-Cells during Mitogen Stimulation for Memory B-Cell ELISpot Analysis Is Influenced by Size and Composition of the B-Cell Pool
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TN_cdi_plos_journals_1547695953
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1547695953
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0102885
