A High-Yield Co-Expression System for the Purification of an Intact Drs2p-Cdc50p Lipid Flippase Comp...
A High-Yield Co-Expression System for the Purification of an Intact Drs2p-Cdc50p Lipid Flippase Complex, Critically Dependent on and Stabilized by Phosphatidylinositol-4-Phosphate
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Author / Creator
Azouaoui, Hassina , Montigny, Cédric , Ash, Miriam-Rose , Fijalkowski, Frank , Jacquot, Aurore , Grønberg, Christina , López-Marqués, Rosa L. , Palmgren, Michael G. , Garrigos, Manuel , le Maire, Marc , Decottignies, Paulette , Gourdon, Pontus , Nissen, Poul , Champeil, Philippe and Lenoir, Guillaume
Publisher
United States: Public Library of Science
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Language
English
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Publisher
United States: Public Library of Science
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Contents
P-type ATPases from the P4 subfamily (P4-ATPases) are energy-dependent transporters, which are thought to establish lipid asymmetry in eukaryotic cell membranes. Together with their Cdc50 accessory subunits, P4-ATPases couple ATP hydrolysis to lipid transport from the exoplasmic to the cytoplasmic leaflet of plasma membranes, late Golgi membranes,...
Alternative Titles
Full title
A High-Yield Co-Expression System for the Purification of an Intact Drs2p-Cdc50p Lipid Flippase Complex, Critically Dependent on and Stabilized by Phosphatidylinositol-4-Phosphate
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Record Identifier
TN_cdi_plos_journals_1624940406
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1624940406
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0112176
