An Integrated System for Precise Genome Modification in Escherichia coli
An Integrated System for Precise Genome Modification in Escherichia coli
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Publisher
United States: Public Library of Science
Journal title
Language
English
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Publisher
United States: Public Library of Science
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Scope and Contents
Contents
We describe an optimized system for the easy, effective, and precise modification of the Escherichia coli genome. Genome changes are introduced first through the integration of a 1.3 kbp Landing Pad consisting of a gene conferring resistance to tetracycline (tetA) or the ability to metabolize the sugar galactose (galK). The Landing Pad is then exci...
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Full title
An Integrated System for Precise Genome Modification in Escherichia coli
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TN_cdi_plos_journals_1710982710
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1710982710
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0136963
