Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)
Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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Contents
Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to the widely used co-immunoprecipitation (co-IP) to identify protein-protein interactions. Here, we used BioID, on its...
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Full title
Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID)
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TN_cdi_plos_journals_1769997463
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1769997463
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0150239
