Optimization of Quantitative PCR Methods for Enteropathogen Detection
Optimization of Quantitative PCR Methods for Enteropathogen Detection
About this item
Full title
Author / Creator
Liu, Jie , Gratz, Jean , Amour, Caroline , Nshama, Rosemary , Walongo, Thomas , Maro, Athanasia , Mduma, Esto , Platts-Mills, James , Boisen, Nadia , Nataro, James , Haverstick, Doris M. , Kabir, Furqan , Lertsethtakarn, Paphavee , Silapong, Sasikorn , Jeamwattanalert, Pimmada , Bodhidatta, Ladaporn , Mason, Carl , Begum, Sharmin , Haque, Rashidul , Praharaj, Ira , Kang, Gagandeep and Houpt, Eric R.
Publisher
United States: Public Library of Science
Journal title
Language
English
Formats
Publication information
Publisher
United States: Public Library of Science
Subjects
More information
Scope and Contents
Contents
Detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. Here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficiency. First, we evaluate the molecular detection and...
Alternative Titles
Full title
Optimization of Quantitative PCR Methods for Enteropathogen Detection
Authors, Artists and Contributors
Author / Creator
Gratz, Jean
Amour, Caroline
Nshama, Rosemary
Walongo, Thomas
Maro, Athanasia
Mduma, Esto
Platts-Mills, James
Boisen, Nadia
Nataro, James
Haverstick, Doris M.
Kabir, Furqan
Lertsethtakarn, Paphavee
Silapong, Sasikorn
Jeamwattanalert, Pimmada
Bodhidatta, Ladaporn
Mason, Carl
Begum, Sharmin
Haque, Rashidul
Praharaj, Ira
Kang, Gagandeep
Houpt, Eric R.
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_plos_journals_1799212519
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1799212519
Other Identifiers
ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0158199
