SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase
SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase
About this item
Full title
Author / Creator
Si, Meng , Xu, Qing , Jiang, Ling and Huang, He
Publisher
United States: Public Library of Science
Journal title
Language
English
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Publisher
United States: Public Library of Science
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Scope and Contents
Contents
SpyTag can spontaneously form a covalent isopeptide bond with its protein partner SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing SpyCatcher at the N terminus and SpyTag at the C terminus. Circular LUC was more thermostable and alkali-tolerant than the wild type, without compromising the specific activity. Struct...
Alternative Titles
Full title
SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase
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Author / Creator
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Record Identifier
TN_cdi_plos_journals_1822391815
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1822391815
Other Identifiers
ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0162318
