Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules
Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules
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Publisher
United States: Public Library of Science
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Language
English
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Publisher
United States: Public Library of Science
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Scope and Contents
Contents
We describe an 8-spot confocal setup for high-throughput smFRET assays and illustrate its performance with two characteristic experiments. First, measurements on a series of freely diffusing doubly-labeled dsDNA samples allow us to demonstrate that data acquired in multiple spots in parallel can be properly corrected and result in measured sample c...
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Full title
Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules
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TN_cdi_plos_journals_1889361413
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_1889361413
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0175766
