Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR
Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR
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United States: Public Library of Science
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Language
English
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Publisher
United States: Public Library of Science
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Contents
A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a CTAB method. The sensitivity of the method was est...
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Full title
Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR
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TN_cdi_plos_journals_2017529223
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2017529223
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0180877
