A simplified workflow for monoclonal antibody sequencing
A simplified workflow for monoclonal antibody sequencing
About this item
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Publisher
United States: Public Library of Science
Journal title
Language
English
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Publication information
Publisher
United States: Public Library of Science
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Scope and Contents
Contents
The diversity of antibody variable regions makes cDNA sequencing challenging, and conventional monoclonal antibody cDNA amplification requires the use of degenerate primers. Here, we describe a simplified workflow for amplification of IgG antibody variable regions from hybridoma RNA by a specialized RT-PCR followed by Sanger sequencing. We perform...
Alternative Titles
Full title
A simplified workflow for monoclonal antibody sequencing
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Record Identifier
TN_cdi_plos_journals_2246222981
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2246222981
Other Identifiers
ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0218717
