Tom20 seems to recognize mainly presequence-containing proteins, whereas Tom70 is specialized for hydrophobic proteins having internal targeting sequences. [...]Tom70 also has a chaperone-like function in preventing precursor proteins from premature folding prior to import [9]. [...]for POMP10, an OM protein of unknown function, pATOM36 was required for membrane insertion [19]. pATOM36 is also present in the tripartite attachment complex (TAC) that connects the single-unit mitochondrial genome of trypanosomes to the basal body of the flagella [18] (Fig 2). [...]to the MIM complex, pATOM36 has a dual function in the biogenesis of OM proteins as well as in the segregation of the replicated mitochondrial genome. [...]pATOM36 has an essential function in OM protein biogenesis and as an essential component of the TAC in the segregation of the replicated single unit kDNA of T. brucei. kDNA, kinetoplast DNA; MIM, mitochondrial import machinery; OM, outer membrane; pATOM36, peripheral ATOM of 36 kDa; TAC, tripartite attachment complex. https://doi.org/10.1371/journal.ppat.1007351.g002 The fungi-specific Mim1 (13 kD) and Mim2 (10 kD) share neither sequence nor structural similarity with the kinetoplastid-specific pATOM36 (36 kD). [...]recent studies have shown that expression of pATOM36 can restore the growth and OM protein biogenesis defects in a yeast strain deleted for both Mim1 and Mim2 [20]. [...]in the converse experim...

Mitochondrial protein import in trypanosomatids: Variations on a theme or fundamentally different?

10.1371/journal.ppat.1007351