An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complemen...
An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation
About this item
Full title
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Publisher
San Francisco: Public Library of Science
Journal title
Language
English
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Publication information
Publisher
San Francisco: Public Library of Science
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Scope and Contents
Contents
PCR-based amplification of annotated genes has allowed construction of expression clones at genome-scale using classical and recombination-based cloning technologies. However, genome-scale expression and purification of proteins for down-stream applications is often limited by challenges such as poor expression, low solubility, large size of multi-...
Alternative Titles
Full title
An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation
Authors, Artists and Contributors
Author / Creator
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Record Identifier
TN_cdi_plos_journals_2426532576
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2426532576
Other Identifiers
ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0235853
