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Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holo...

Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holo...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2452939314

Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holotrichia oblita

About this item

Full title

Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holotrichia oblita

Publisher

United States: Public Library of Science

Journal title

PloS one, 2020-10, Vol.15 (10), p.e0240972

Language

English

Formats

Publication information

Publisher

United States: Public Library of Science

More information

Scope and Contents

Contents

Quantitative real-time polymerase chain reaction (qPT-PCR) is commonly used to analyze gene expression, however, the accuracy of the normalized results is affected by the expression stability of reference genes. Holotrichia oblita (Coleoptera: Scarabaeidae) causes serious damage to crops. Reliable reference genes in H. oblita are needed for qRT-PCR...

Alternative Titles

Full title

Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holotrichia oblita

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_plos_journals_2452939314

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2452939314

Other Identifiers

ISSN

1932-6203

E-ISSN

1932-6203

DOI

10.1371/journal.pone.0240972

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