Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
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Author / Creator
Yale IMPACT Research Team , Kudo, Eriko , Israelow, Benjamin , Vogels, Chantal B. F. , Lu, Peiwen , Wyllie, Anne L. , Tokuyama, Maria , Venkataraman, Arvind , Brackney, Doug E. , Ott, Isabel M. , Petrone, Mary E. , Earnest, Rebecca , Lapidus, Sarah , Muenker, M. Catherine , Moore, Adam J. , Casanovas-Massana, Arnau , Omer, Saad B. , Dela Cruz, Charles S. , Farhadian, Shelli F. , Ko, Albert I. , Grubaugh, Nathan D. and Iwasaki, Akiko
Publisher
United States: Public Library of Science
Journal title
Language
English
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Publication information
Publisher
United States: Public Library of Science
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Scope and Contents
Contents
The current quantitative reverse transcription PCR (RT-qPCR) assay recommended for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) testing in the United States requires analysis of 3 genomic targets per sample: 2 viral and 1 host. To simplify testing and reduce the volume of required reagents, we devised a multiplex RT-qPCR assay to de...
Alternative Titles
Full title
Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
Authors, Artists and Contributors
Author / Creator
Kudo, Eriko
Israelow, Benjamin
Vogels, Chantal B. F.
Lu, Peiwen
Wyllie, Anne L.
Tokuyama, Maria
Venkataraman, Arvind
Brackney, Doug E.
Ott, Isabel M.
Petrone, Mary E.
Earnest, Rebecca
Lapidus, Sarah
Muenker, M. Catherine
Moore, Adam J.
Casanovas-Massana, Arnau
Omer, Saad B.
Dela Cruz, Charles S.
Farhadian, Shelli F.
Ko, Albert I.
Grubaugh, Nathan D.
Iwasaki, Akiko
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_plos_journals_2460094906
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2460094906
Other Identifiers
ISSN
1545-7885,1544-9173
E-ISSN
1545-7885
DOI
10.1371/journal.pbio.3000867