RNA-Seq is not required to determine stable reference genes for qPCR normalization
RNA-Seq is not required to determine stable reference genes for qPCR normalization
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United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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Assessment of differential gene expression by qPCR is heavily influenced by the choice of reference genes. Although numerous statistical approaches have been proposed to determine the best reference genes, they can give rise to conflicting results depending on experimental conditions. Hence, recent studies propose the use of RNA-Seq to identify sta...
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Full title
RNA-Seq is not required to determine stable reference genes for qPCR normalization
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TN_cdi_plos_journals_2640120422
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2640120422
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ISSN
1553-7358,1553-734X
E-ISSN
1553-7358
DOI
10.1371/journal.pcbi.1009868