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RNA-Seq is not required to determine stable reference genes for qPCR normalization

RNA-Seq is not required to determine stable reference genes for qPCR normalization

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2640120422

RNA-Seq is not required to determine stable reference genes for qPCR normalization

About this item

Full title

RNA-Seq is not required to determine stable reference genes for qPCR normalization

Publisher

United States: Public Library of Science

Journal title

PLoS computational biology, 2022-02, Vol.18 (2), p.e1009868-e1009868

Language

English

Formats

Publication information

Publisher

United States: Public Library of Science

More information

Scope and Contents

Contents

Assessment of differential gene expression by qPCR is heavily influenced by the choice of reference genes. Although numerous statistical approaches have been proposed to determine the best reference genes, they can give rise to conflicting results depending on experimental conditions. Hence, recent studies propose the use of RNA-Seq to identify sta...

Alternative Titles

Full title

RNA-Seq is not required to determine stable reference genes for qPCR normalization

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_plos_journals_2640120422

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2640120422

Other Identifiers

ISSN

1553-7358,1553-734X

E-ISSN

1553-7358

DOI

10.1371/journal.pcbi.1009868

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