Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation effici...
Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency
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Publisher
United States: Public Library of Science
Journal title
Language
English
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Publisher
United States: Public Library of Science
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Scope and Contents
Contents
Engineering improved B-family DNA polymerases to catalyze 3'-O-modified nucleotide reversible terminators is limited by an insufficient understanding of the structural determinants that define polymerization efficiency. To explore the key mechanism for unnatural nucleotide incorporation, we engineered a B-family DNA polymerase from Thermococcus Kod...
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Full title
Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency
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TN_cdi_plos_journals_3166996171
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_3166996171
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0316531
