Method for Quantitating the Molecular Content of a Subcellular Organelle: Hormone and Neurophysin Co...
Method for Quantitating the Molecular Content of a Subcellular Organelle: Hormone and Neurophysin Content of Newly Formed and Aged Neurosecretory Granules
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Publisher
United States: National Academy of Sciences of the United States of America
Journal title
Language
English
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Publisher
United States: National Academy of Sciences of the United States of America
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Scope and Contents
Contents
A method is described for the quantitative determination of the content of subcellular organelles such as secretory granules. Purified subcellular fractions of the organelle are prepared and aliquots are assayed for hormones, for example. To determine the number of organelles per fraction, known numbers of latex particles of a size similar to the o...
Alternative Titles
Full title
Method for Quantitating the Molecular Content of a Subcellular Organelle: Hormone and Neurophysin Content of Newly Formed and Aged Neurosecretory Granules
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Record Identifier
TN_cdi_pnas_primary_81_1_180_fulltext
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pnas_primary_81_1_180_fulltext
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.81.1.180
