Double Targeted Gene Replacement for Creating Null Mutants
Double Targeted Gene Replacement for Creating Null Mutants
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Publisher
United States: National Academy of Sciences of the United States of America
Journal title
Language
English
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Publisher
United States: National Academy of Sciences of the United States of America
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Scope and Contents
Contents
We have used double gene targeting to create homozygous gene replacements in the protozoan parasite Leishmania major, an asexual diploid. This method uses two independent selectable markers in successive rounds of gene targeting to replace both alleles of an endogenous gene. We developed an improved hygromycin B-resistance cassette encoding hygromy...
Alternative Titles
Full title
Double Targeted Gene Replacement for Creating Null Mutants
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Author / Creator
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Record Identifier
TN_cdi_pnas_primary_88_16_7170_fulltext
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pnas_primary_88_16_7170_fulltext
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.88.16.7170
