A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Prote...
A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Proteins
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United States: National Academy of Sciences
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Language
English
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United States: National Academy of Sciences
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Contents
GFP and the red fluorescent protein, DsRed, have been combined to design a protease assay that allows not only for fluorescence resonance energy transfer (FRET) studies but also for dual-color crosscorrelation analysis, a single-molecule-based method that selectively probes the concomitant movement of two distinct tags. The measurement principle is...
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Full title
A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Proteins
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TN_cdi_pnas_primary_99_19_12161
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pnas_primary_99_19_12161
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ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.192433499