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A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Prote...

A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Prote...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pnas_primary_99_19_12161

A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Proteins

About this item

Full title

A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Proteins

Publisher

United States: National Academy of Sciences

Journal title

Proceedings of the National Academy of Sciences - PNAS, 2002-09, Vol.99 (19), p.12161-12166

Language

English

Formats

Publication information

Publisher

United States: National Academy of Sciences

More information

Scope and Contents

Contents

GFP and the red fluorescent protein, DsRed, have been combined to design a protease assay that allows not only for fluorescence resonance energy transfer (FRET) studies but also for dual-color crosscorrelation analysis, a single-molecule-based method that selectively probes the concomitant movement of two distinct tags. The measurement principle is...

Alternative Titles

Full title

A Protease Assay for Two-Photon Crosscorrelation and FRET Analysis Based Solely on Fluorescent Proteins

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pnas_primary_99_19_12161

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pnas_primary_99_19_12161

Other Identifiers

ISSN

0027-8424

E-ISSN

1091-6490

DOI

10.1073/pnas.192433499

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