Multispot single-molecule FRET: high-throughput analysis of freely diffusing molecules
Multispot single-molecule FRET: high-throughput analysis of freely diffusing molecules
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Publisher
Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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Language
English
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Publisher
Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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Contents
We describe an 8-spot confocal setup for high-throughput smFRET assays and illustrate its performance with two characteristic experiments. First, measurements on a series of freely diffusing doubly-labeled dsDNA samples allow us to demonstrate that data acquired in multiple spots in parallel can be properly corrected and result in measured sample c...
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Full title
Multispot single-molecule FRET: high-throughput analysis of freely diffusing molecules
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TN_cdi_proquest_journals_2070156297
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2070156297
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E-ISSN
2692-8205
DOI
10.1101/085027
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https://www.proquest.com/docview/2070156297?pq-origsite=primo&accountid=13902
