Apoptosis-inducing effect of Tanshinone and its molecular mechanism on human lung cancer cells
Apoptosis-inducing effect of Tanshinone and its molecular mechanism on human lung cancer cells
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Tianjin: Chinese Anti-Cancer Association Chinese Antituberculosis Association
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Chinese
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Tianjin: Chinese Anti-Cancer Association Chinese Antituberculosis Association
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目的 了解丹参酮对人肺癌细胞(SPC-A-1)的凋亡诱导作用,并探讨其可能的分子机理.方法 体外培养的SPC-A-1细胞经无毒剂量的丹参酮ⅡA(0.5 mg/L)处理5天,光镜、电镜观察细胞凋亡情况,流式细胞仪检测凋亡指数及凋亡相关基因的蛋白表达,并以全反式维甲酸及顺铂作为对照.结果 SPC-A-1细胞经丹参酮ⅡA处理后,电镜观察可见多量凋亡细胞.流式细胞仪检测:丹参酮组凋亡指数显著高于顺铂组及对照组,而与维甲酸组比较无显著差异.丹参酮组促凋亡基因p53、Fas、Bax表达水平明显升高,而凋亡抑制基因Bcl-2的表达水平则显著降低.结论 丹参酮ⅡA具有诱导肺癌SPC-A-1细胞凋亡的作用,其分子机理可能是上调p53、Bax、Fas及下调Bcl-2的表达. Objective To study...
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Apoptosis-inducing effect of Tanshinone and its molecular mechanism on human lung cancer cells
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TN_cdi_proquest_journals_2127758996
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2127758996
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ISSN
1009-3419
E-ISSN
1999-6187
DOI
10.3779/cjlc.v5i4.2962
