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Functional study of spermatozoa derived from neonatal mouse testes grafted into immunodeficient mice...

Functional study of spermatozoa derived from neonatal mouse testes grafted into immunodeficient mice...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2182417462

Functional study of spermatozoa derived from neonatal mouse testes grafted into immunodeficient mice / 新生小鼠睾丸组织异体异位移植物中精子形态及功能的研究

About this item

Full title

Functional study of spermatozoa derived from neonatal mouse testes grafted into immunodeficient mice / 新生小鼠睾丸组织异体异位移植物中精子形态及功能的研究

Publisher

Xi'an: Xi'an Jiaotong University

Journal title

Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban, 2006-01 (3), p.220

Language

Chinese

Formats

Publication information

Publisher

Xi'an: Xi'an Jiaotong University

More information

Scope and Contents

Contents

目的采用卵胞浆内单精子显微注射(ICSI)技术探讨新生小鼠睾丸组织异体异位移植到裸鼠体内后所生成精子的受精能力.方法将含有原始生精细胞的新生1-2 d小鼠睾丸组织异体异位移植到BALB/c-nu/nu免疫缺陷小鼠背部皮下.8周后取出移植物,通过常规染色和电镜技术对生精小管中生精细胞组成和形态进行观察;以薄层明胶水解法检测精子顶体蛋白酶活性;采用ISCI技术检测移植物中具有成熟形态精子的受精能力.结果移植物的HE染色发现,新生小鼠睾丸组织在裸鼠体内已启动并进行精子发生过程,生精小管中含有包括精子在内的所有类型生精细胞;移植物组织经处理后,可计数到具有镰刀头状的长尾精子约1×103个/mg移植物;透射电镜标本中观察到的精原细胞和精母细胞均具有正常超微结构;薄层明胶水解法检测可观察到顶体蛋白酶呈阳...

Alternative Titles

Full title

Functional study of spermatozoa derived from neonatal mouse testes grafted into immunodeficient mice / 新生小鼠睾丸组织异体异位移植物中精子形态及功能的研究

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_journals_2182417462

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2182417462

Other Identifiers

ISSN

1671-8259

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