Construction of expression vector for NT4-ADNF-9 fusion gene / NT4-ADNF-9融合基因原核表达载体的构建
Construction of expression vector for NT4-ADNF-9 fusion gene / NT4-ADNF-9融合基因原核表达载体的构建
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Full title
Construction of expression vector for NT4-ADNF-9 fusion gene / NT4-ADNF-9融合基因原核表达载体的构建
Publisher
Xi'an: Xi'an Jiaotong University
Journal title
Xi'an jiao tong da xue xue bao. Journal of Xi'an Jiaotong University (medical sciences). Yi xue ban, 2003-01 (5), p.427
Language
Chinese
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Publication information
Publisher
Xi'an: Xi'an Jiaotong University
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Scope and Contents
Contents
目的构建神经营养素(NT4)与活性依赖性神经营养因子-9(ADNF-9)融合基因的原核表达载体pBV220/NT4-ADNF-9,为进一步对神经系统退行性疾病基因治疗的研究奠定基础. 方法采用非对称互补引物/模板法,制备两端含有酶切位点的ADNF-9的cDNA,将其连接到NT的信号肽和前导序列的3′端,组成融合基因NT4-ADNF-9,再将该融合基因亚克隆于原核表达载体pBV220,构建为pBV220/NT4-ADNF-9. 结果经DNA测序,限制性内切酶酶切等方法证实已成功地将ADNF-9重组到NT4信号肽和前导序列的3′端,并将融合基因亚克隆于pBV220内. 结论成功构建了NT4与ADNF-9融合基因的原核表达载体pBV220/NT4-ADNF-9.
Alternative Titles
Full title
Construction of expression vector for NT4-ADNF-9 fusion gene / NT4-ADNF-9融合基因原核表达载体的构建
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Record Identifier
TN_cdi_proquest_journals_2182447339
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2182447339
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ISSN
1671-8259
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