Sox2 is a master transcriptional regulator of embryonic development. In this study, we determined the protein interactome of Sox2 in the chromatin and nucleoplasm of mouse embryonic stem (mES) cells. Apart from canonical interactions with pluripotency regulating transcription factors, we identified interactions with several chromatin modulators, including members of the heterochromatin protein 1 (HP1) family, suggesting a role of Sox2 in transcriptional repression. Sox2 was also found to interact with RNA binding proteins. RNA immunoprecipitation followed by sequencing revealed that Sox2 associates with small nucleolar RNA Snord34 and with the non-coding RNA 7SK. 7SK has been shown to regulate transcription at regulatory regions, which could suggest a functional interaction with Sox2 for chromatin recruitment. Nevertheless, we found no evidence of Sox2 modulating recruitment of 7SK to chromatin when examining 7SK chromatin occupancy by Chromatin Isolation by RNA Purification (ChIRP) in Sox2 depleted mES cells. In addition, knockdown of 7SK in mES cells did not lead to any change in Sox2 occupancy at 7SK-regulated genes. Thus, our results suggest that Sox2 and 7SK co-exist in a ribonucleoprotein complex whose function is not to regulate chromatin recruitment, but might rather regulate other processes in the nucleoplasm. Footnotes * https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-7570 * https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-7640...

Interaction of Sox2 with RNA binding proteins in mouse embryonic stem cells

10.1101/560383