Over the course of the past decade, tissue clearing methods have reached a high level of sophistication with a wide variety of approaches now available. To image large cleared samples, light-sheet microscopes have proven to be ideal due to their excellent optical sectioning capability in transparent tissue. Such instruments have recently seen extensive technological and commercial development. However, despite this progress, the community is lacking instruments capable of exploring large samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy (mesoSPIM) initiative, an open-hardware project that provides researchers with instructions and software to easily build and operate light-sheet microscopes for centimeter-sized cleared samples (www.mesospim.org)....

The mesoSPIM initiative: open-source light-sheet mesoscopes for imaging in cleared tissue

10.1101/577122